The influence of three different solvents on estimation of sumatriptan from bulk and pharmaceutical dosage forms was studied. The solutions of sumatriptan were prepared with the water and the solvent blends containing water: acetonitrile (1:1) and water: methanol (1:1). The solutions were scanned with in the ranges of 200 nm to 400nm. Sumatriptan exhibited max at 283 nm in case of water, however the max was slightly shifted to 283.5 nm with the solvents acetonitrile: water and methanol: water. The method obeyed Beer-Lamberts law with in the range of 10 to 50 g/ml concentration. The analytical parameters such as linearity, A (1%, 1 cm), LOD, LOQ, inter day precision, intraday precision, molar absorption coefficient, and sandells sensitivity were calculated. The results of analysis were validated by recovery studies. The recovery was more than 98%. The method was found to be simple, accurate, precise, economical and robust. The validation parameters were treated statistically with ANOVA and there were significant differences noticed. This study clearly indicated that the solvent is having any influence in determination of sumatriptan from bulk drug as well as from the formulations. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of sumatriptan succinate from pharmaceutical preparation.
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